文献类型     学术论文
标    题     Definition of subclasses of nucleoplasmic RNA
著    者     IGOR TAMM
机    构    The Rockefeller University
集合名     Proc. Nati. Acad. Sci. USA
年 1977
月 11
卷 74
期 11
地    域     New York, USA
文件大小     1.13 MB
描    述     RNA synthesis in isolated HeLa cell nuclei prepared from cells pretreated with 5,6-dichloro-I-P-D-ribofu- ranosylbenzimidazole (DRB) is inhibited in a time-dependent manner. After 40-min pretreatment of cells with 60B M DRB in the presence of actinomycin D (0.04 ,g/ml), the rate of RNA synthesis in isolated nuclei, measured by[3HJUTP incorpora- tion, is decreased by 63%. The DRB-resistant one-third of het- erogeneous nuclear is distributed over the entire size range of heterogeneous nuclear RNA ...More

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11PHYS1002260203_13466470