文献类型     学术论文
标    题     Interferon messenger RNA content of human fibroblasts during induction, shutoff, and superinduction of interferon production
著    者     PRAVINKUMAR B. SEHGAL; BERNHARD DOBBERSTEIN; IGOR TAMM
机    构     The Rockefeller University
主题词     human diploid fibroblast cells; poly(I)poly(C); McAuslan-Tomkins translational repressor hypothesis; 5,6-dichloro-l-l-D- ribofuranosylbenzimidazole; Xenopus laevis oocytes
集合名     Proc. Natl. Acad. Sci. USA
年 1977
月 8
卷 74
期 8
地    域     New York, New York
文件大小     1.17 MB
描    述     Translation of injected mRNA in oocytes of Xenopus laevis has been used as a highly sensitive and quanti- tative assay for interferon mRNA. Injection into oocytes of polyadenylylated RNA extracted from poly(I)poly(C)induced human diploid fibroblasts (FS-4) leads to the synthesis of bio- logically active human fibroblast interferon over a period of 24-32 hr. There is a linear relationship between the amount of mRNA injected and the interferon yield obtained over a range of 1-20 ng of injected RNA...More

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